Dataset Curation (WIP)

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Dataset Curation (WIP)#

Users are always encouraged to evaluate ROI’s after any processing run. There are a few differing approaches depending on the experimental question.

These approaches fall on two sides of a spectrum:

  • Some users opt for an “accept everything” approach which sets all thresholds low enough to pass ROI criterion and use post-hoc correlation measures to narrow down cell counts.

  • Other users use parameters that ideally get ~95% accuracy, and bridge the remaining ~5% through manually accepting and rejecting the misses.

Which approach you take may depend on the experimental question. Do you care about sparesely active cells, i.e. cells that may have 1 significant firing event over the course of the entire session? If so, you may wish to “accept everything”

Trace Activity

Summary Images#

Suite2p gives you several summary images to use when deciding accepted/rejected cells.

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