3. Explained: Axial Offset Correction#
3.1. Background#
Light beads traveling to our sample need to be temporally distinct relative to our sensor so that the aquisition system knows the origin and subsequent depth of each bead.
The current LBM design incoorperates 2 cavities, hereby named Cavity A and Cavity B.
These two cavities are non-overlapping areas where light beads travel. If we plot
a sample pollen grain through each z-depth, we can see these cavities manifest:
We see a bi-modal distribution of Signal (Y) vs z-depth.
This pollen grain is sampled just like a brain would be sampled. We can preview the time-series resulting from this pollen to get a preliminary look at our recording quality:
