I/O

1. I/O#

Functions to help with loading and saving data.

Write a supported lazy imaging array to disk.

This function handles writing multi-dimensional imaging data to various formats, with support for ROI selection, z-plane registration, chunked streaming, and format conversion. Use with imread() to load and convert imaging data.

Parameters:

lazy_arrayobject

One of the supported lazy array readers providing .shape, .metadata, and _imwrite() methods:

MboRawArray : Raw ScanImage/ScanMultiROI TIFF files with phase correction
Suite2pArray : Memory-mapped binary (data.bin or data_raw.bin) + ops.npy
MBOTiffArray : Multi-file TIFF reader using Dask backend
TiffArray : Single or multi-TIFF reader
H5Array : HDF5 dataset wrapper (h5py.File[dataset])
ZarrArray : Collection of z-plane .zarr stores
NumpyArray : Single .npy memory-mapped NumPy file
NWBArray : NWB file with “TwoPhotonSeries” acquisition dataset

outpathstr or Path

Target directory to write output files. Will be created if it doesn’t exist. Files are named automatically based on plane/ROI (e.g., plane01_roi1.tiff).

extstr, default=”.tiff”

Output format extension. Supported formats: - .tiff, .tif : Multi-page TIFF (BigTIFF for >4GB) - .bin : Suite2p-compatible binary format with ops.npy metadata - .zarr : Zarr v3 array store - .h5, .hdf5 : HDF5 format

planeslist | tuple | int | None, optional

Z-planes to export (1-based indexing). Options: - None (default) : Export all planes - int : Single plane, e.g. planes=7 exports only plane 7 - list/tuple : Specific planes, e.g. planes=[1, 7, 14]

roiint | Sequence[int] | None, optional

ROI selection for multi-ROI data. Options: - None (default) : Stitch/fuse all ROIs horizontally into single FOV - 0 : Split all ROIs into separate files (one file per ROI per plane) - int > 0 : Export specific ROI, e.g. roi=1 exports only ROI 1 - list/tuple : Export specific ROIs, e.g. roi=[1, 3]

num_framesint, optional

Number of frames to export. If None (default), exports all frames.

register_zbool, default=False

Perform z-plane registration using Suite3D before writing.

shift_vectorsnp.ndarray, optional

Pre-computed z-shift vectors with shape (n_planes, 2) for [dy, dx] shifts.

metadatadict, optional

Additional metadata to merge into output file headers/attributes.

overwritebool, default=False

Whether to overwrite existing output files.

orderlist | tuple, optional

Reorder planes before writing. Must have same length as planes.

target_chunk_mbint, optional

Target chunk size in MB for streaming writes. Default is 100 MB.

progress_callbackCallable, optional

Callback function for progress updates: callback(progress, current_plane).

debugbool, default=False

Enable verbose logging for troubleshooting.

output_namestr, optional

Filename for binary output when ext=”.bin”.

output_suffixstr, optional

Custom suffix to append to output filenames. If None (default), files are named with “_stitched” for multi-ROI data when roi is None, or “_roiN” for specific ROIs. Examples: “_stitched”, “_processed”, “_session1”. The suffix is automatically sanitized (illegal characters removed, double extensions prevented, underscore prefix added if missing).

**kwargs

Additional format-specific options passed to writer backends.

Returns:

Path: Path to the output directory containing written files.

Examples

>>> from mbo_utilities import imread, imwrite
>>> data = imread("path/to/raw/*.tiff")
>>> imwrite(data, "output/session1", roi=None)  # Stitch all ROIs

>>> # Save specific planes
>>> imwrite(data, "output/session1", planes=[1, 7, 14])

>>> # Split ROIs
>>> imwrite(data, "output/session1", roi=0)

>>> # Z-plane registration
>>> imwrite(data, "output/registered", register_z=True)

>>> # Convert to Suite2p binary
>>> imwrite(data, "output/suite2p", ext=".bin", roi=0)

>>> # Save to Zarr
>>> imwrite(data, "output/zarr_store", ext=".zarr")

mbo_utilities.imread(inputs: str | Path | ndarray | Sequence[str | Path], **kwargs)[source]#

Lazy load imaging data from supported file types.

Currently supported file types: - .bin: Suite2p binary files (.bin + ops.npy) - .tif/.tiff: TIFF files (BigTIFF, OME-TIFF and raw ScanImage TIFFs) - .h5: HDF5 files - .zarr: Zarr v3 - .npy: NumPy arrays - np.ndarray: In-memory numpy arrays (wrapped as NumpyArray)

Parameters:

inputsstr, Path, ndarray, or sequence of str/Path: Input source. Can be: - Path to a file or directory - List/tuple of file paths - A numpy array (will be wrapped as NumpyArray for full imwrite support) - An existing lazy array (passed through unchanged)
**kwargs: Extra keyword arguments passed to specific array readers.

Returns:

array_like: One of Suite2pArray, TiffArray, MboRawArray, MBOTiffArray, H5Array, ZarrArray, NumpyArray, or IsoviewArray.

Examples

>>> from mbo_utilities import imread, imwrite
>>> arr = imread("/data/raw")  # directory with supported files
>>> arr = imread("data.tiff")  # single file
>>> arr = imread(["file1.tiff", "file2.tiff"])  # multiple files

>>> # Wrap numpy array for imwrite compatibility
>>> data = np.random.randn(100, 512, 512)
>>> arr = imread(data)  # Returns NumpyArray
>>> imwrite(arr, "output", ext=".zarr")  # Full write support

mbo_utilities.get_files(base_dir, str_contains='', max_depth=1, sort_ascending=True, exclude_dirs=None) → list | Path[source]#

Recursively search for files in a specified directory whose names contain a given substring, limiting the search to a maximum subdirectory depth. Optionally, the resulting list of file paths is sorted in ascending order using numeric parts of the filenames when available.

This function intelligently handles zarr stores: it stops recursing into leaf .zarr directories (those that don’t contain nested .zarr subdirs) to avoid traversing thousands of internal chunk directories.

Parameters:

base_dirstr or Path: The base directory where the search begins. This path is expanded (e.g., ‘~’ is resolved) and converted to an absolute path.
str_containsstr, optional: A substring that must be present in a file’s name for it to be included in the result. If empty, all files are matched.
max_depthint, optional: The maximum number of subdirectory levels (relative to the base directory) to search. Defaults to 1. If set to 0, it is automatically reset to 1.
sort_ascendingbool, optional: If True (default), the matched file paths are sorted in ascending alphanumeric order. The sort key extracts numeric parts from filenames so that, for example, “file2” comes before “file10”.
exclude_dirsiterable of str or Path, optional: An iterable of directories to exclude from the resulting list of file paths. By default will exclude “.venv/”, “__pycache__/”, “.git” and “.github”].

Returns:

list of str: A list of full file paths (as strings) for files within the base directory (and its subdirectories up to the specified depth) that contain the provided substring.

Raises:

FileNotFoundError: If the base directory does not exist.
NotADirectoryError: If the specified base_dir is not a directory.

Examples

>>> import mbo_utilities as mbo
>>> # Get all files that contain "ops.npy" in their names by searching up to 3 levels deep:
>>> ops_files = mbo.get_files("path/to/files", "ops.npy", max_depth=3)
>>> # Get only files containing "tif" in the current directory (max_depth=1):
>>> tif_files = mbo.get_files("path/to/files", "tif")

mbo_utilities.files_to_dask(files: list[str | Path], astype=None, chunk_t=250)[source]#

Lazily build a Dask array or list of arrays depending on filename tags.

“plane”, “z”, or “chan” → stacked along Z (TZYX)
“roi” → list of 3D (T,Y,X) arrays, one per ROI
otherwise → concatenate all files in time (T)

mbo_utilities.get_metadata(file, dx: float | None = None, dy: float | None = None, dz: float | None = None, verbose: bool = False, z_step: float | None = None)[source]#

Extract metadata from a TIFF file or directory of TIFF files produced by ScanImage.

This function handles single files, lists of files, or directories containing TIFF files. When given a directory, it automatically finds and processes all TIFF files in natural sort order. For multiple files, it calculates frames per file accounting for z-planes.

Parameters:

fileos.PathLike, str, or list

Single file path: processes that file
Directory path: processes all TIFF files in the directory
List of file paths: processes all files in the list

dxfloat, optional

X pixel resolution in micrometers. Overrides extracted value.

dyfloat, optional

Y pixel resolution in micrometers. Overrides extracted value.

dzfloat, optional

Z step size in micrometers. Overrides extracted value. Also available as z_step for backward compatibility.

verbosebool, optional

If True, returns extended metadata including all ScanImage attributes. Default is False.

z_stepfloat, optional

Alias for dz (backward compatibility).

Returns:

dict

A dictionary containing extracted metadata with normalized resolution aliases: - dx, dy, dz: canonical resolution values in micrometers - pixel_resolution: (dx, dy) tuple - voxel_size: (dx, dy, dz) tuple - umPerPixX, umPerPixY, umPerPixZ: Suite2p format - PhysicalSizeX, PhysicalSizeY, PhysicalSizeZ: OME format

For multiple files, also includes: - ‘frames_per_file’: list of frame counts per file (accounting for z-planes) - ‘total_frames’: total frames across all files - ‘file_paths’: list of processed file paths - ‘tiff_pages_per_file’: raw TIFF page counts per file

Raises:

ValueError: If no recognizable metadata is found or no TIFF files found in directory.

Examples

>>> # Single file with z-resolution
>>> meta = get_metadata("path/to/rawscan_00001.tif", dz=5.0)
>>> print(f"Voxel size: {meta['voxel_size']}")

>>> # Directory of files
>>> meta = get_metadata("path/to/scan_directory/")
>>> print(f"Files processed: {len(meta['file_paths'])}")
>>> print(f"Frames per file: {meta['frames_per_file']}")

>>> # List of specific files
>>> files = ["scan_00001.tif", "scan_00002.tif", "scan_00003.tif"]
>>> meta = get_metadata(files, dz=5.0)

mbo_utilities.expand_paths(paths: str | Path | Sequence[str | Path]) → list[Path][source]#

Expand a path, list of paths, or wildcard pattern into a sorted list of actual files.

This is a handy wrapper for loading images or data files when you’ve got a folder, some wildcards, or a mix of both.

Parameters:

pathsstr, Path, or list of (str or Path): Can be a single path, a wildcard pattern like “*.tif”, a folder, or a list of those.

Returns:

list of Path: Sorted list of full paths to matching files.

Examples

>>> expand_paths("data/\*.tif")
[Path("data/img_000.tif"), Path("data/img_001.tif"), ...]

>>> expand_paths(Path("data"))
[Path("data/img_000.tif"), Path("data/img_001.tif"), ...]

>>> expand_paths(["data/\*.tif", Path("more_data")])
[Path("data/img_000.tif"), Path("more_data/img_050.tif"), ...]

mbo_utilities.get_mbo_dirs() → dict[source]#

Ensure ~/mbo and its subdirectories exist.

Returns a dict with paths to the root, settings, and cache directories.

I/O

Contents

1. I/O#